jcla.23771.pdf (554.9 kB)
Stability and validity of intact parathyroid hormone levels in different sample types and storage conditions
journal contribution
posted on 2023-06-10, 00:02 authored by Haitham Khalil, Anwar Borai, Mohammed Dakhakhni, Suhad Bahijri, Hala Faizo, Fawzi F Bokhari, Gordon FernsGordon Ferns, Ahmed A MirzaBackground Several pre-analytical factors can affect the measurement of intact Parathyroid Hormone (IPTH). In this study, we have investigated the effects of using different types of tubes, time elapsed before separation, and storage conditions over time on the measured values of IPTH. Method Blood samples from 30 subjects were collected into plain, SST, and EDTA tubes. All serum and plasma were separated immediately (first set) and after 2 hrs delay (second set). The first set of samples were aliquoted and stored at RT (25°C), at fridge (4°C), and freezer (-20°C). IPTH was measured in all the stored aliquots at 2,4, and 8 days after collection using Architect analyzer. Results Paired T test and ANOVA repeated measures showed no significant difference between IPTH levels in all tubes. The second set of serum and plasma were significantly lower (3.8% and 7.4%, p < 0.001, respectively) when compared to samples measured initially. Serum samples stored at RT were significantly lower (by 45%,59%, and 77%) on days 2,4, and 8 when compared to the initial time (p < 0.001 in all cases). Plasma samples stored at RT, were significantly lower on day 8 after collection, by 30.8% (p < 0.001). These differences would be clinically important. Conclusion Plasma IPTH can be stored at RT for up to four days. Both plasma and serum IPTH are not affected by a delay in the separation of up to two h and they can be stored for up to 8 days in a fridge or freezer without any clinically significant changes in their values.
History
Publication status
- Published
File Version
- Published version
Journal
Journal of Clinical Laboratory AnalysisISSN
0887-8013Publisher
WileyExternal DOI
Page range
1-8Article number
a23771Event location
United StatesDepartment affiliated with
- Division of Medical Education Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2021-06-04First Open Access (FOA) Date
2021-06-04First Compliant Deposit (FCD) Date
2021-06-04Usage metrics
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