Synthesis, structural and biological studies of ubiquitin mutants containing (2S, 4S)-5-fluoroleucine residues strategically placed in the hydrophobic core

Alexeev, Dmitriy; Barlow, Paul N; Bury, Stella M; Charrier, Jean-Damien; Cooper, Alan; Hadfield, David; Jamieson, Craig; Kelly, Sharon M; Layfield, Robert; Mayer, R John; McSparron, Helen; Price, Nicholas C; Ramage, Robert; Sawyer, Lindsay; Starkmann, Bernard A; Uhrin, Dusan; Wilken, Jill; Young, Douglas W

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Synthesis, structural and biological studies of ubiquitin mutants containing (2S, 4S)-5-fluoroleucine residues strategically placed in the hydrophobic core

journal contribution

posted on 2023-06-07, 20:31 authored by Dmitriy Alexeev, Paul N Barlow, Stella M Bury, Jean-Damien Charrier, Alan Cooper, David Hadfield, Craig Jamieson, Sharon M Kelly, Robert Layfield, R John Mayer, Helen McSparron, Nicholas C Price, Robert Ramage, Lindsay Sawyer, Bernard A Starkmann, Dusan Uhrin, Jill Wilken, Douglas W Young

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History

Publication status

Published

Journal

ChemBioChem

ISSN

1439-4227

Publisher

Wiley

External DOI

https://doi.org/10.1002/cbic.200300699

Issue

9

Volume

4

Page range

894-896

Pages

3.0

Department affiliated with

Chemistry Publications

Notes

DWY is corresponding author and directed the synthetic work. The work reports the first ubiquitin variants in which selected leucine residues in the hydrophobic core have been replaced by (2S,4S)-5-fluoroleucine residues. Protein X-ray crystallographic, 19F-NMR and biophysical methods were used to show that protein folding was unafected.

Full text available

No

Peer reviewed?

Yes

Legacy Posted Date

2012-02-06

File(s) not publicly available

Synthesis, structural and biological studies of ubiquitin mutants containing (2S, 4S)-5-fluoroleucine residues strategically placed in the hydrophobic core

History

Publication status

Journal

ISSN

Publisher

External DOI

Issue

Volume

Page range

Pages

Department affiliated with

Notes

Full text available

Peer reviewed?

Legacy Posted Date

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