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The Zipper Region of Epstein-Barr Virus bZIP Transcription Factor Zta Is Necessary but Not Sufficient To Direct DNA Binding
journal contributionposted on 2023-06-07, 23:09 authored by Matthew R. Hicks, Salama S. Al-Mehairi, Alison Sinclair
The viral bZIP transcription factor Zta (BZLF1, EB1, ZEBRA) mediates the switch between the latent and lytic cycles of Epstein-Barr virus (EBV). In part, its activity requires the formation of homodimers and interaction with specific DNA sequence elements (ZREs). Zta has an atypical zipper motif that has a lower stability than do typical bZIP proteins. Here we show that a synthetic peptide directed against the zipper can disrupt the DNA-binding function of Zta. This highlights the relevance of this region for the function of Zta and demonstrates that the zipper region is a potential target for therapeutic agents. We also unmask the relevance of a region adjacent to the zipper (CT region), which is required to direct the interaction of Zta with DNA and to transactivate ZRE-dependent promoters in vivo.
JournalJournal of Virology
PublisherAmerican Society for Microbiology
Department affiliated with
- Biochemistry Publications
NotesThis research was entirely undertaken in Dr Sinclair's group at the University of Sussex. The work identifies the functional relevance of a novel region of a viral transcription factor.
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