Houghton_Microbiol Spectrum_2021.pdf (1018.39 kB)
The mycobacterium tuberculosis sRNA F6 modifies expression of essential chaperonins, GroEL2 and GroES
journal contribution
posted on 2023-06-10, 01:11 authored by Joanna Houghton, Angela Rodgers, Graham Rose, Alexandre D’Halluin, Terry Kipkorir, Declan Barker, Simon WaddellSimon Waddell, Kristine B ArnvigAlmost 140?years after the identification of Mycobacterium tuberculosis as the etiological agent of tuberculosis, important aspects of its biology remain poorly described. Little is known about the role of posttranscriptional control of gene expression and RNA biology, including the role of most of the small RNAs (sRNAs) identified to date. We have carried out a detailed investigation of the M. tuberculosis sRNA F6 and shown it to be dependent on SigF for expression and significantly induced in starvation conditions in vitro and in a mouse model of infection. Further exploration of F6 using an in vitro starvation model of infection indicates that F6 affects the expression of the essential chaperonins GroEL2 and GroES. Our results point toward a role for F6 during periods of low metabolic activity typically associated with long-term survival of M. tuberculosis in human granulomas. IMPORTANCE Control of gene expression via small regulatory RNAs (sRNAs) is poorly understood in one of the most successful pathogens, Mycobacterium tuberculosis. Here, we present an in-depth characterization of the sRNA F6, including its expression in different infection models and the differential gene expression observed upon deletion of the sRNA. Our results demonstrate that deletion of F6 leads to dysregulation of the two essential chaperonins GroEL2 and GroES and, moreover, indicate a role for F6 in the long-term survival and persistence of M. tuberculosis in the human host.
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Publication status
- Published
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- Published version
Journal
Microbiology SpectrumISSN
2165-0497Publisher
American Society for MicrobiologyExternal DOI
Issue
2Volume
9Page range
1Article number
a01095-21Department affiliated with
- Global Health and Infection Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2021-09-30First Open Access (FOA) Date
2021-09-30First Compliant Deposit (FCD) Date
2021-09-29Usage metrics
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