File(s) not publicly available
The protein kinase CK2 facilitates repair of chromosomal DNA single-strand breaks
journal contributionposted on 2023-06-08, 06:48 authored by Joanna I Loizou, Sherif F El-Khamisy, Anastasia Zlatanou, David J Moore, Douglas W Chan, Jun Qin, Stefania Sarno, Flavio Meggio, Lorenzo A Pinna, Keith CaldecottKeith Caldecott
CK2 was the first protein kinase identified and is required for the proliferation and survival of mammalian cells. Here, we have identified an unanticipated role for CK2. We show that this essential protein kinase phosphorylates the scaffold protein XRCC1 and thereby enables the assembly and activity of DNA single-strand break repair protein complexes in vitro and at sites of chromosomal breakage. Moreover, we show that inhibiting XRCC1 phosphorylation by mutation of the CK2 phosphorylation sites or preventing CK2 activity using a highly specific inhibitor ablates the rapid repair of cellular DNA single-strand breaks by XRCC1. These data identify a direct role for CK2 in the repair of chromosomal DNA strand breaks and in maintaining genetic integrity.
Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
NotesWork was conducted in my laboraotory, by my student (JL)- External authors provided some materials. Identified a novel role for casein kinase in DNA repair.
Full text available