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X-Ray Diffraction Studies of Amyloid Structure
journal contribution
posted on 2023-06-07, 22:33 authored by O Sumner Makin, Louise SerpellLouise SerpellElucidation of the underlying core structure of amyloid fibrils is essential for understanding the mechanism by which amyloid fibrils are formed and deposited. Conventional methods of X-ray crystallography and NMR cannot be used, since the fibers are insoluble and heterogeneous. X-ray fiber diffraction is one method that has been successfully used to examine the structure of these insoluble fibers. The procedure involves the formation of suitable, ordered amyloid fibrils and characterization (by electron microscopy), partial alignment of fibers, X-ray data collection, data analysis, and finally, model building.
History
Publication status
- Published
Journal
Methods in Molecular BiologyISSN
1064-3745External DOI
Volume
299Page range
67-80Pages
14.0Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes