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A wide field time resolved fluorescent lifetime imaging microscope

posted on 2023-06-09, 03:11 authored by Phil BirchPhil Birch, L Moore, X Li, R Phillips, T Conneely
Fluorescence lifetime imaging microscopy (FLIM) is a tool that has become of important use to many biologists and medical researchers. It allows for the imaging of fluorescent markers within cells and as well their decay lifetime information. This lifetime information yields useful information about the chemical properties of the local region surrounding the fluorophore marker. It is therefore desirable to be able to measure this information and localise it to produce either a 2D or 3D image stack of the lifetimes and intensity information. Two major problems must be overcome to achieve good resolution, both spatially and temporally. There must be a method to provide optical depth sectioning and the imaging must be fast. Sectioning can be done with confocal or two photon microscopes however, these are point scanning devices and are relatively slow. We will detail a new microscope system based on light sheet illumination that uses a micro channel plate (MCP) device called a Capacitive Division Imaging Readout (CDIR) which has been developed by Photek Ltd. The device uses an array of capacitors to move the charge site from the MCP to four pre-amplifiers and time- over-threshold discriminators. This camera has the ability to image photons as well as measure the arrival time, enabling high speed FLIM imaging of biological samples. The optical sectioning is achieved by using a diffraction limited sheet of light, that illuminates the sample from the side in the plane orthogonal to the objectives optical axis. We will present the first results taken from the microscope.


Publication status

  • Published

Presentation Type

  • paper

Event name

Photon 16

Event location

Leeds, UK

Event type


Event date

5-8 September 2016

Department affiliated with

  • Engineering and Design Publications

Full text available

  • No

Peer reviewed?

  • No

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