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Translesion synthesis in mammalian cells
DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialized low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five polymerases used in TLS in human cells have different substrate specificities, enabling them to deal with many different types of damaged bases. PCNA plays a central role in recruiting the TLS polymerases and effecting the polymerase switch from replicative to TLS polymerase. When the fork is blocked PCNA gets ubiquitinated. This increases its affinity for the TLS polymerases, which all have novel ubiquitin-binding motifs, thereby facilitating their engagement at the stalled fork to effect TLS.
History
Publication status
- Published
Journal
Experimental Cell ResearchISSN
0014-4827Publisher
ElsevierExternal DOI
Issue
14Volume
312Page range
2673-2676Full text available
- Yes
Peer reviewed?
- Yes